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KMID : 0620920180500080094
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Experimental & Molecular Medicine
2018 Volume.50 No. 8 p.94 ~ p.94
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CircularRNA_104670 plays a critical role in intervertebral disc degeneration by functioning as a ceRNA
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Song Jian
Wang Hong-Li
Song Ke-Han
Ding Zhi-Wen
Wang Hai-Lian
Ma Xiao-Sheng
Lu Fei-Zhou
Xia Xin-Lei
Wang Ying-Wei
Fei-Zou
Jiang Jian-Yuan
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Abstract
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This study was carried out to explore the roles of circular RNAs (circRNAs) in nucleus pulposus (NP) tissues in intervertebral disc degeneration (IDD). Differentially expressed circRNAs in IDD and normal NP tissues were identified based on the results of microarray analysis. Bioinformatics techniques were employed to predict the direct interactions of selected circRNAs, microRNAs (miR), and mRNAs. CircRNA_104670 was selected as the target circRNA due to its large multiplier expression in IDD tissues. After luciferase reporter and EGFP/RFP reporter assays, we confirmed that circRNA_104670 directly bound to miR-17-3p, while MMP-2 was the direct target of miR-17-3p. The receiver-operating characteristic (ROC) curve showed that circRNA_104670 and miR-17-3p had good diagnostic significance for IDD (AUC circRNA_104670?=?0.96; AUC miRNA-17-3p?=?0.91). A significant correlation was detected between the Pfirrmann grade and expression of circRNA_104670 (r?=?0.63; p?=?0.00) and miR-17-3p (r?=??0.62; p?=?0.00). Flow-cytometric analysis and the MTT assay showed that interfering with circRNA_104670 using small interfering RNA (siRNA) inhibited NP cell apoptosis (p?
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KEYWORD
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Mechanisms of disease, RNA sequencing
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